Electrophysiological Detection of Non-IgE-mediated Hypersensitivity to the Fragrance Eugenol (2-Methoxy–4-(2-propenyl)phenol, MW 164 D) in Human Rectal Biopsies In Vitro

Non-IgE-mediated hypersensitivity to fragrances cannot be measured by skin-prick tests or specific serum-IgE (RAST) tests, and the diagnosis is based on epicutaneous patch tests, which have a poor sensitivity and/or specificity. We examined rectal biopsies of 2 patients with an atopic condition, who had a history of intolerance to spices and foods containing eugenol, and compared them to biopsies of 6 other atopic patients without a history of eugenol intolerance. Four to 6 rectal biopsies per patient were mounted in Ussing chambers (serosal surface area 1.1mm2) with carbogenated Ringer’s solution at 37o C. Shor…