Cellular immunotherapy in late stage breast cancer patients with reactivated autologous Memory T-cells derived from bone marrow

Tumorspecific Memory T-cells (MTC) can be found in the bone marrow (BM) in the majority of primary and metastatic breast cancer (BC) patients by using ELISpot-analysis. Upon specific restimulation with tumourantigen-pulsed dendritic cells (DC) autologous T-cells exert specific effector functions like IFN-gamma production and cytotoxicity. Furthermore we have shown in NOD/Scid-mice that reactivated MTC are able to infiltrate autologous and heterologous tumor tissue, proliferate and kill tumor cells by induction of apoptosis, leading to a marked or complete tumor rejection within 21 days after transfer (Nature Med…