CareLit Fachartikel
The Endocannabinoid 2-Arachidonoyl Glycerol Induces Apoptosis in Primary Hepatic Stellate Cells and Is Upregulated During Hepatic Fibrogenesis
SV Siegmund, T Qian, J Harvey-White, G Kunos, DA Brenner, RF Schwabe · Zeitschrift für Gastroenterologie · 2006 · Heft 01
Dokument
1040662
CareLit-ID
Jahr
2006
Publikation
PDF
nein
Metadaten
DOI
ja
zitierfähig
Bibliografische Angaben
Zusammenfassung
Aims: The endogenous cannabinoid 2-arachidonoyl glycerol (2-AG) is a lipid mediator that blocks proliferation and induces cell death in many cell types. AIM: To determine 2-AG levels during hepatic fibrogenesis and evaluate its effect on primary hepatic stellate cells (HSCs) and hepatocytes. Methods: Primary rat hepatocytes and rat and human HSCs were isolated from healthy human or rat liver by collagenase perfusion. 2-AG-induced cell death was analyzed by LDH assay and western blot for caspase 3- and PARP-cleavage. Reactive oxygen species (ROS) formation was monitored by DCFDA fluorescence and confocal microsco…
Schlagworte
2-Arachidonoyl Glycerol
Anti-Fibrotic Therapy
Cell Death
Endocannabinoids
Hepatic Stellate Cells
Hepatocytes
Zeitschrift für Gastroenterologie
