Human Precision Cut Liver Tumor Slices as a comprehensive predictive test system for the oncolytic effectiveness of measles vaccine viruses
M Zimmermann, S Armeanu, I Smirnow, S Kupka, S Wagner, M Wehrmann, MG Rots, GMM Groothuis, T Weiß, A Königsreiner, M Gregor, M Bitzer, UM Lauer · Zeitschrift für Gastroenterologie · 2008 · Heft 09
Bibliografische Angaben
Zusammenfassung
Availability of an individualized preselection of oncolytic viruses to be used for virotherapy of GI tumor patients would be of great help. Using primary liver tumor resection specimens we evaluated the Precision Cut Liver Slice (PCLS) technology as a novel in vitro test system for characterization of paramount tumor infection parameters of individual patients. Primary human liver tumor resection material of 20 well-characterized donors exhibiting primary (8 HCC, 3 CC) or secondary (9 CRC) tumors of the liver, were cut in 200–300µm thick tissue slices using the Krumdieck™ tissue slicer. The PCLS were cultivated…
