The miR-17–92 gene cluster is amplified and overexpressed in retinoblastoma
E Biewald, P Mestdagh, H Stephan, K de Preter, T Thor, M Baumann, D Lohmann, A Eggert, F Spelemann, A Schramm, J Vandesompele, JH Schulte · Klinische Pädiatrie · 2009 · Heft 05
Bibliografische Angaben
Zusammenfassung
Aberrant microRNA expression is involved in the pathogenesis and progression of human malignancies, including embryonal tumors. Retinoblastoma is a common eye tumor of childhood and is mainly driven by mutations in the Rb1-gene. Genomic alterations observed in retinoblastoma also include amplification of chromosome 6p22. Neither high-resolution CGH nor miRNA expression have been investigated in primary retinoblastomas to date. We here used high throughput real-time PCR to analyze the expression of 430 microRNAs in 30primary retinoblastomas in comparison to normal human retina. In addition, we used aCGH to analys…
