Expression of galectins and the Thomsen-Friedenreich antigen in normal, IUGR, preeclamptic and HELLP placentas – Galectin–1, Galectin–3 and TF expression

Aims: Galectin–1 (gal–1) and galectin–3 (gal–3), which are members of the mammalian β-galactoside-binding proteins, recognize preferentially Galβ1–4GlcNAc sequences of several cell surface oligosaccharides. In addition, gal–1 binds also to the Thomsen-Friedenreich (TF) antigen (Galβ1–3GalNAc-). Material and Methods: Slides of frozen and paraffin embedded placental tissue of patients with IUGR, preeclamptic patients, HELLP patients and normal term placentas were incubated with monoclonal and polyclonal antibodies against gal–1, gal–3 and TF. Staining reaction was performed with the ABC reagent. Intensity of immun…