Proteome analysis of cerebrospinal fluid in patients with multiple sclerosis

Background: Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of unknown cause. Although diagnostic criteria for early MS are available, there is still a need for biomarkers predicting disease subtype and progression. Therefore, we had analyzed the cerebrospinal fluid (CSF) proteome profile of MS patients by 2-dimensional difference in-gel electrophoresis (2-D-DIGE) technology. Our previous results showed clear differences in the proteome profile of patients with RRMS and CIS as compared to normal controls. For validation of these findings and to evaluate the diagnostic sensitivity and spec…