Assessment of the interaction between presenilin 1 (PS1) and ubiquilin 1 splice variants in intact cells

Background/ojective: Ubiquilin 1 splice variants are differentially expressed in the brains of Alzheimer patients. We have previously shown that endogenous presenilin 1 (PS1) and ubiquilin 1 come into close proximity in murine primary neurons. The objective of this study was to determine whether PS1 comes into close proximity with each of the different ubiquilin 1 splice variants and whether there are differences in their interactions. Methods: Chinese Hamster Ovary (CHO) cells were co-transfected with a wild type PS1 construct and either one of the four, v5-tagged ubiquilin 1 splice variants (TV1-V5, TV2-V5, TV…