PREPARATION OF IRON OXIDE LABELLED PRIMARY HUMAN HEPATOCYTES SUITABLE FOR CELL TRANSPLANTATION
N Raschzok, H Morgul, R Schwartlander, N Billecke, C Leist, FWR Vondran, N Kammer, L Stelter, L Stelter, J Pinkernelle, J Pinkernelle, U Teichgraber, U Teichgraber, I Sauer · Zeitschrift für Gastroenterologie · 2008 · Heft 01
Bibliografische Angaben
Zusammenfassung
Aim: Transplantation of primary human hepatocytes is a promising approach for treatment of certain liver diseases. For a better understanding of the processes during and following transplantation, visualisation of the transplanted cells is desirable. Aim was the preparation of iron oxide labelled primary human hepatocytes for cell transplantation and the evaluation of this treatment on viability and metabolic activity of the cells. Methods: Primary human hepatocytes were isolated from liver specimen obtained from patients undergoing partial hepatectomy. Cells were incubated with fluorescence-labelled, micron-siz…