Bile acids induce intracellular acidosis and ATP depletion in human colonic crypts
E Kunstár, K Farkas, Z Rakonczay Jr, F Nagy, T Molnár, Z Szepes, T Takács, V Venglovecz, T Wittmann, P Hegyi · Zeitschrift für Gastroenterologie · 2010 · Heft 05
Bibliografische Angaben
Zusammenfassung
Introduction: Under pathophysiological conditions, bile acids can reach the large intestine in high concentrations and can induce watery diarrhoea. This could be due to reduced Cl- or Na+ uptake or stimulated fluid and Cl- secretion. Aims: Our aim was to investigate the effects of bile acids on human colonic epithelial cells. Methods: Colonic biopsies were obtained from patients with negative colonoscopic finding. After collagenase digestion, the isolated colonic crypts were loaded with the pH sensitive fluorescent dye BCECF. The intracellular ATP level (ATPi) was determined using Mg-green dye which has been sho…
