Trypsin inhibits pancreatic ductal bicarbonate secretion via CFTR Cl-channel

Background: Intraacinar activation of pancreatic enzymes is a key event in the pathogenesis of acute pancreatitis. Activated enzymes entering into the ductal system may influence the fluid and bicarbonate secretion of pancreatic ductal cells. Therefore, the aim of this study was to investigate the effects of trypsin on pancreatic ductal bicarbonate secretion. Methods: Pancreatic protease-activated receptor 2 (PAR2) expression was determined by immunohistochemistry in guinea pigs. Intact intra/interlobular pancreatic ducts and single pancreatic duct cells were isolated from the pancreas of guinea pigs. The activi…