Gene-metabolite networks for signature of insulin action in humans

Objective: Insulin is the major metabolic hormone with multilevel and systemic effect on the body. Few studies describe insulin-dependent transcriptome changes and no data exist for insulin-related plasma metabolome. We investigated the regulation of subcutaneous adipose tissue (SAT) gene expression during different glucose/insulin concentrations and its relation with insulin-induced changes of plasma metabolome. Design and methods: Healthy subjects (n=14) underwent a saline-infusion test (INF) and/or a hyperinsulinemic-euglycemic clamp (EC), and/or a hyperinsulinemic-hyperglycemic clamp (HC). SAT biopsy and pla…