Expression profiles of human adipocyte differentiation in the SGBS cell model

Current information on transcriptional control of adipocyte differentiation is mainly derived from mouse model systems. We differentiated human preadipocytes of the SGBS cell line into mature adipocytes and characterized the alterations in gene expression patterns with whole genome microarrays. 248 genes were significantly (fdr 0.05) altered more than 5-fold during the differentiation process. Amongst these genes we recovered well known markers of adipogenesis such as adiponectin, C/EBPα or PPARγ. Altogether 31% of the upregulated genes were associated with lipid metabolism. Principal component analysis correctl…