Liver Sinusoidal Endothelial Cells inhibit inflammatory cytokine responses of CD4 T cells through skewed accesory signals

Liver cells are able to down-regulate the inflammatory activity of infiltrating immune cells, however, the cellular and molecular activity behind this capability is unclear. Here, we investigated whether liver sinusoidal endothelial cells (LSEC) could abrogate inflammatory cytokine secretion by CD4 T cells. We induced inflammatory CD4 T cells by stimulating murine splenic CD4 T cells with dendritic cells (DC) and antibody to CD3. Upon restimulation, DC-primed CD4 T cells secreted high amounts of interferon-g (IFNg; (>1000 pg/ml) and IL17 (100 pg/ml). The DC-primed CD4 T cells were then restimulated twice either…