A functional role of Macrophage Migration Inhibitory Factor (MIF) for liver steatosis in mice by activation of AMP kinase

Background: The cytokine macrophage migration inhibitory factor (MIF) is known to be involved in various inflammatory diseases. We have recently discovered an unexpected anti-fibrotic function of MIF in experimental mouse models which is mediated by activation of the stress kinase AMPK (Heinrichs et al. PNAS 2011). AMPK is also known to be involved in lipogenesis. We therefore assessed the specific role of MIF in hepatic steatosis in vivo and in vitro. Methods: Male MIF knockout (Mif -/-) and wild-type mice were fed a high fat (60% fat/kcal, HFD) or a methionine and choline-deficient diet (MCD). Weight gain was…