Molecular analysis of Gremlin in gliomas
LJ Isselstein, M Gessi, T Müller, D Luxen, D Freihoff, J Freihoff, A Becker, M Simon, T Pietsch, A Waha · Klinische Pädiatrie · 2012 · Heft 04
Bibliografische Angaben
Zusammenfassung
Gremlin was identified as a regulator of growth and development in Xenopus laevis and a transcript downregulated in mos transformed rat cells. Hypermethylation and expression of Gremlin was studied in gliomas by pyrosequencing and realtime RT-PCR in 63 gliomas, 6 GBM cell lines and 7 stem cell enriched primary GBM cell cultures. Structural alterations were investigated by SNP analysis and high resolution melting studies. The capacity of focus formation was studied on transiently transfected GBM cells. Gliomas showed remarkably reduced mRNA-levels of Gremlin and excessive hypermethylation compared to normal brain…
