Potential role of FP receptors in human and rat hepatocytes for the development of hepatic insulin resistance

Introduction: Recent studies revealed a potential role of prostanoids in the development of hepatic insulin resistance and non-alcoholic fatty liver disease (NAFLD). The work centered around PGE2, because microsomal PGE-synthase (mPGES) was strongly induced early in the course of experimental NAFLD models. Nevertheless, other prostaglandins, in particular PGF2α, are also formed in liver and hence might impact on disease development. Methods: Primary cultures rat hepatocytes were preincubated with PGF2α, FP-receptor selective agonists or antagonists and subsequently stimulated with Insulin. Phosphorylation of com…