The sulfation level of glycosaminoglycans in monocytes and macrophages

Sulfation of glycosaminoglycans (GAGs) of components of extracellular matrix and cell surface is an important regulatory mechanism in inflammatory immune response. All sulfotransferases use 3′-phosphoadenosine 5′-phosphosulfate (PAPS) as a common sulfate donor that is produced by PAPS synthase. In order to detect the sulfation level of monocytes/macrophages we established a CD44-hyaluronan binding assay based on flow cytometry. Monocytes were isolated from human peripheral blood of healthy volunteers. Macrophages were cultivated from monocytes at 37 °C in the presence of 100 U/ml granulocytes/macrophages – colon…