“In vivo” shRNA screen for the identification of the RUNX1/ETO targets with a relevance in leukaemogenesis

The t(8;21) translocation results in the expression of the oncogenic fusion protein RUNX1/ETO. This transcription factor affects transcriptional regulation predisposing to leukaemia. To interrogate RUNX1/ETO partner and target genes for their involvement in leukaemic propagation, we performed an shRNA screen. This screen contained shRNAs against up-regulated (PTK2, ANGPT1) as well as down-regulated (CEBP/α) direct and indirect target genes as well as genes previously suggested to cooperate with RUNX1/ETO (RUVBL1, CAPNS1). Parallel screens were performed in vitro and in a xenotransplantation model. Whereas RUNX1/…