Strain specific differences in the effects of TGF-beta on hepatocyte viability and CYP450 activity in mice

Primary hepatocytes represent a widely used experimental system in the field of toxicology research and drug studies. In this study, we compared hepatocytes from mouse inbred strains C57/BL6 and DBA/2J. These two lines are the parental strains of the BXD recombinant inbred strain panel. We performed a detailed comparison of isolated hepatocytes of the two strains for susceptibility to transforming growth factor (TGF)-beta exposure and induced effects. We detected strain specific differences upon TGF-beta exposure, namely a significant decrease in the viable cell number within five days of culture and an earlier…