Effect of Estrogen Receptor β agonists on proliferation and gene expression of ovarian cancer cells

Estrogen receptor (ER) b affects ovarian carcinogenesis. We examined the effects of four ERβ agonists on proliferation and gene expression of OVCAR3 and OAW-42 cells. OVCAR3 and OAW-42 ovarian cancer cells were treated with the ERβ agonists ERB-041, WAY200070, Liquiritigenin and 3β-Adiol and cell growth was measured by means of the Cell Titer Blue Assay (Promega). Proliferation was examined after knockdown of ERβ using specific siRNA. Transcriptome analyses were performed with these cell lines after treatment with ERB-041, WAY200070 and Liquiritigenin by means of Affymetrix GeneChip arrays. After treatment of OV…