Development of a human organotypic tumor invasion model of cancer cell line MDA-MB-231 in Precision-Cut Lung Slices (PCLS)

Cancer such as breast carcinoma is a major public health problem worldwide. The actual cause of death is the formation of metastasis which often occurs in the lung. Among the cell types present in the tumor microenvironment, macrophages have been proven to be the dominant leukocyte population with high macrophage density correlating to poor patient prognosis. Thus, these cells are of high interest as targets for cancer therapeutics, making them a valuable research topic. We utilize an innovative organotypic tumor invasion model, using living human Precision-Cut Lung Slices (PCLS) and cancer cells to focus on the…