Cathepsin D expression in pancreatic ductal adenocarcinoma (PDAC) cells increases proliferation and reduces survival of pancreatic cancer patients
U Mahajan, E Langhoff, E Costello, W Greenhalf, C Halloran, T Schwaiger, G Beyer, F Weiss, J Neoptolemos, T Kohlmann, M Maron, M Büchler, M Lerch, J Mayerle · Zeitschrift für Gastroenterologie · 2015 · Heft 08
Bibliografische Angaben
Zusammenfassung
Background: Remodelling of the pancreatic cancer tumour microenvironment and extracellular matrix (ECM) has been implicated in the prognosis of PDAC. We investigated whether the aspartic protease cathepsin-D contributes to proliferation, migration and fibrogenesis of pancreatic cancer and whether its expression affects the survival of pancreatic cancer patients. Material & Methods: Murine PSC and pancreatic cancer PD6606-cells were used for WB and IF. Cathepsin-D activity was inhibited with pepstatin and its expression silenced with siRNA. TGFβ1 was measured by ELISA and proliferation by MTT assay. Tumour tissue…
