Isolation of primary human hepatocytes from human liver tissue after portal vein embolization
M Kluge, N Raschzok, A Reutzel-Selke, H Napierala, KH Hillebrandt, RD Major, B Strücker, A Leder, J Siefert, P Tang, S Lippert, H Sallmon, D Seehofer, J Pratschke, IM Sauer · Zeitschrift für Gastroenterologie · 2015 · Heft 12
Bibliografische Angaben
Zusammenfassung
Primary human hepatocytes are an important resource for basic research, pharmaceutical testing, and therapeutic concepts in regenerative medicine. Primary human hepatocytes can be isolated from resected liver tissue. Preoperative portal vein embolization (PVE) is increasingly used to decrease the risk of delayed postoperative liver regeneration by induction of selective hypertrophy of the future remnant liver tissue. We here investigate the effect of PVE on the outcome of hepatocyte isolation. Primary human hepatocytes were isolated from liver tissue obtained from partial hepatectomies (n = 190) using the two-st…
