CareLit Fachartikel

Efficacy of the BH3-mimetic ABT-199 in acute lymphoblastic leukemia

R Hörl, F Seyfried, S Demir, J Zinngrebe, S Köhrer, KM Debatin, LH Meyer · Klinische Pädiatrie · 2016 · Heft 05

Dokument
1054831
CareLit-ID
Jahr
2016
Publikation
PDF
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Metadaten
DOI
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Bibliografische Angaben

Zeitschrift
Klinische Pädiatrie
Autor:innen
R Hörl, F Seyfried, S Demir, J Zinngrebe, S Köhrer, KM Debatin, LH Meyer
Ausgabe
Heft 05 / 2016
Seiten
–
Erschienen: 2016-05-02 00:00:00
ISSN
0300-8630
DOI
10.1055/s-0036-1582495

Zusammenfassung

Introduction: Acute lymphoblastic leukemia (ALL) cells can evade from apoptosis by upregulating anti-apoptotic proteins. Mcl-1 and Bcl-2 are frequently overexpressed in ALL, thus, appearing as promising targets for anti-ALL therapy. Methods: In vitro/ex vivo exposure of cell lines and primary patient-derived xenograft samples (pdx) to ABT-199, Dinaciclib and chemotherapy, assessment of cellular viability, generation of Mcl-1 knockout cell lines by CRISPR/Cas9, Western blot, NOD/SCID human ALL mouse model. Results: 4/6 cell lines and 14/16 pdx samples were highly sensitive to ABT-199. Resistance was associated wi…

Schlagworte

Klinische Pädiatrie