Isolation of circulating tumor cells and subsequent analysis for PIK3CA mutation using CellSearch-CellCelector workflow
MHD Neumann, Y Decker, H Schneck, A Franken, S Schömer, T Fehm, H Neubauer, D Niederacher · Senologie - Zeitschrift für Mammadiagnostik und -therapie · 2016 · Heft 04
Bibliografische Angaben
Zusammenfassung
Circulating tumor cells (CTC) are rare cells which have successfully dissociated from the primary tumor into the blood stream and could lead to metastases. Isolation of CTCs is necessary to understand heterologous CTC biology. Here we show a workflow to characterize CTCs by combining the CellSearch®- and CellCelector™-System. Single cells were classified as CTCs if nucleus (DAPI) and cytokeratin (FITC and/or TRITC) positive while CD45 (Cy5) negative. Detection and quantification of single cells from control samples and patient samples were performed using CellSearch- and CellCelector-System. CTCs were isolated f…
