Longitudinal analysis of cystic fibrosis of airways' microbiota

In the last decades, complexity of cystic fibrosis airways microbiota just started to get explored. However, most previous studies used cross-sectional designs thus obtaining snapshots of the lung microbiota at one time point. A longitudinal approach is needed to explore the stability of the CF microbiota over time. In this study, we collected and analyzed 389 samples from the lower airways microbiota (sputum and/or throat swabs) of 91 CF patients (0 to 70 yo) over a long timeframe (4 – 34 samples over 2 years). The microbiota was then explored by 16S amplicons sequencing. We observe a high diversity in the micr…