How to isolate and characterize EpCAMnegative circulating tumor cells in metastatic breast cancer patients
R Lampignano, M Neumann, B Behrens, D Köhler, D Niederacher, T Fehm, NH Stoecklein, H Neubauer · Geburtshilfe und Frauenheilkunde · 2016 · Heft 10
Bibliografische Angaben
Zusammenfassung
Circulating tumor cells (CTCs) are epithelial cells identified in the peripheral blood stream of solid tumors and are believed to be their metastatic precursors. CTCs are mostly detected by EpCAM-based systems that miss those cells which acquired mesenchymal features and believed to be the most malignant ones. In this project we established a workflow combining the Parsortix© system and the CellCelector™micromanipulator to enrich for EpCAM-negative CTCs by size and to isolate them for further molecular characterizations. A cohort of 21 metastatic breast cancer blood samples depleted for EpCAM-positive CTCs was p…
