Differential effects of primary and immortalized hepatocytes on liver mesenchymal cells in co-cultures
J Dudas, V Bordoni, R Novosyadlyy, JG Scharf, A Elmaouhoub, I Aprigliano, B Saile, M Tripodi, G Ramadori · Zeitschrift für Gastroenterologie · 2004 · Heft 02
Bibliografische Angaben
Zusammenfassung
Aims: Hepatocytes are in tight contact with the hepatic stellate cells (HSC) of the liver sinusoid and with the myofibroblasts (rMF) of the pericentral and perivenous area. Hepatocyte-derived signals may influence the behaviour of these cells. Methods: HSC, rMF and hepatocytes (HC) were isolated from rat liver. HSC and rMF were co-cultured with primary HC or with met-murine hepatocytes (immortalized, untransformed cell line, MMH) using cell culture inserts (transwell culture), or were treated with MMH or HC conditioned media for two days. Co-cultured and medium treated HSC and rMF were analysed by cell counting,…
