Analysis of lens protein after extracapsular cataract surgery

Purpose: Post-translational modifications of lens proteins play a crucial role in the formation of cataract during ageing. Samples were obtained from diabetic and non-diabetic patients after extracapsular cataract surgery. Cadaver lenses were used as control. Methods: Samples were homogenized and stored in a buffered medium containing protease inhibitor. Soluble and insoluble protein fractions were obtained by centrifugation at 13,000 x g for 30 minutes. Total protein concentrations were measured by the Bradford method. The electrophoretic separation was performed in a 12.5% SDS/PAG according to Laemmli. Silver…