Immunohistochemical Detection of Specific S100a13 Immunoreactivity in Middle Ear Cholesteatoma

Objective: To conduct a comparative morphologic study on expression of S100a13, a novel member of Ca2+-binding proteins superfamily, in middle ear cholesteatoma and meatal skin. Material and Methods: 5 middle ear cholesteatoma samples and the corresponding external auditory canal skin specimens were subjected to indirect immunofluorescence using a polyclonal monospecific anti-S100a13 serum, developed in our laboratory. Laryngeal carcinoma samples were used as a positive control in this study. Results: Specific S100? 13 immunoreactivity was revealed in all layers of the cholesteatoma matrix and subepithelial gran…