TNF-α, IL-8 and CD14 gene polymorphisms in Helicobacter pylori-positive duodenal ulcer patients

Background: The activation of inflammatory cytokines plays an important role in the pathogenesis of the development of Helicobacter pylori (HP)-positive duodenal ulcer (DU). Cytokine production is determined in part by the nucleotide polymorphisms of cytokine genes. The aim was to evaluate the frequency of TNF-α, IL-8 and CD14 gene polymorphisms in HP-positive DU patients. Patients. 69 patients with HP-positive DU (mean age 46.8 years) and 47 HP- positive healthy blood donors (mean age 42.6 years) were included in the study. Methods: The TNFα-308A (G to A) gene polymorphism was determined by NcoI RFLP (restricti…