Differentially expressed genes in mouse liver during development of fatty liver disease (FLD)

FLD is considered as the hepatic manifestation of the metabolic syndrome but still poorly understood at the molecular level. To study differentially expressed genes during development of FDL in C57BL/6 mice upon challenge with a high fat diet for 1 and 5 weeks, respectively. Hepatic gene expression was determined in livers from 3 groups of four male mice. Total liver RNA was isolated, reverse transcribed and labelled with Cy5- or Cy3-dUTP, respectively. Labeled probes were hybridized to the same microarray spotted with more than 17,000 oligonucleotides. Slides were scanned with a GENE PIX 4000B array scanner and…