P53 determination in intestinal metaplasia by immunohistochemistry, chip and conventional sequencing technology

Background: The mutations of p53 gene play an important role in the pathogenesis of malignant diseases. Data about localization of specific p53 mutations are not well known in intestinal metaplasia. Aims: Our aim was to examine if there were any differences between the DNA isolated from blood, from the altered tissue biopsy and from the healthy part of the stomach. Our aims were also to find disease specific p53 mutations, and to find relation between the results of immunohistochemistry and sequencing data. To compare the traditional sequencing technique with DNA sequencing microarray technology is also an impor…