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Properties of cryopreserved fetal liver stem/progenitor cells in vitro and long-term repopulation of the normal rat liver after cell transplantation

A Menthena, M Oertel, YQ Chen, DA Shafritz · Zeitschrift für Gastroenterologie · 2006 · Heft 01

Dokument
1072528
CareLit-ID
Jahr
2006
Publikation
PDF
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Metadaten
DOI
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Bibliografische Angaben

Zeitschrift
Zeitschrift für Gastroenterologie
Autor:innen
A Menthena, M Oertel, YQ Chen, DA Shafritz
Ausgabe
Heft 01 / 2006
Seiten
–
Erschienen: 2006-01-16 00:00:00
ISSN
0044-2771
DOI
10.1055/s-2006-931716

Zusammenfassung

Cell transplantation is a promising alternative to liver transplantation. Previously, we showed a level of long-term liver replacement by embryonic day (ED)14 fetal liver stem/progenitor cells that should be sufficient for most clinical applications. In this study, we investigated the properties of fetal liver cells after cryopreservation. Using simultaneous analysis for AFP and CK19, freshly isolated unfractionated fetal liver cells showed 2.4% of the cells to be positive for both AFP and CK19. The percentage of viable AFP+/CK19+ cells after cryopreservation (1–18 months) and thawing was maintained at 2.3%. To…

Schlagworte

fetal liver stem/progenitor cells cryopreservation cell culture cell transplantation liver repopulation Zeitschrift für Gastroenterologie