High frequency of anti-PDC-E1a/E1b antibodies in sera from primary biliary cirrhosis patients: affinity purification of the mitochondrial M4 antigen predominantly enriches pyruvat…
CP Berg, GM Stein, R Klein, M Pascu, T Berg, W Kammer, M Priemer, A Nordheim, K Schulze-Osthoff, M Gregor, S Wesselborg, PA Berg · Zeitschrift für Gastroenterologie · 2006 · Heft 01
Bibliografische Angaben
Zusammenfassung
Objective: Sera from patients with primary biliary cirrhosis (PBC) contain antibodies against intracellular antigens such as denatured epitopes of the pyruvate dehydrogenase complex (PDC) derived from bovine heart mitochondria (M2). In addition, native proteins were detected within the sucrose gradient fractions derived from rat liver mitochondria (M4) only by the complement fixation test but not in Western Blot analysis. Thus, the major objective of this study was to identify these proteins. Methods: IgG fractions of M4-CFT positive (n=5) or negative (n=5) PBC marker sera were used to purify the M4 proteins by…
