Pneumococci induced IL-8 expression in human pulmonary epithelium by TLR1 and TLR2 and Rac1
B Schmeck, S Huber, K Moog, J Zahlten, A Hocke, B Opitz, S Hammerschmidt, T Mitchell, M Kracht, S Rosseau, N Suttorp, S Hippenstiel · Pneumologie · 2006 · Heft 02
Bibliografische Angaben
Zusammenfassung
Introduction: Streptococcus pneumoniae is the major pathogen of community-acquired pneumonia. The respiratory epithelium constitutes the first line of defense against invading lung pathogens, including pneumococci. We analyzed the involvement of toll-like receptors and Rho-GTPase signaling in the activation of human lung epithelial cells by pneumococci. Methods: BEAS-2B cells, transient transfection, IL-8 ELISA, chromatin-immunoprcipitation. Results: S. pneumoniae induced release of IL-8 by human bronchial epithelial cell line BEAS-2B. Specific inhibition of Rac1 by Nsc23766 or dominant-negative mutants of Rac1…
