Invasive glioma cells remodel their guiding blood vessel: in vivo microscopy reveals strategies for effective tumour dissemination

Dissemination of cancer cells determines the dim prognosis of many malignancies. Malignant gliomas are characterized by highly invasive cells that extensively infiltrate the brain along fibre tracts and blood vessels, compromising treatment options. Up to today it has been difficult to image infiltrating glioma cells deep in the living brain over time in high resolution, which would allow better understanding of the pathophysiology, molecular determinants, and effectiveness of therapeutics. Here we propose a novel methodology by combining in vivo multiphoton laser scanning microsopy (MPLSM) with the chronic cran…