Detection of reactive oxygen species in a stroke-model of the isolated perfused rat brain

Reactive oxygen species (ROS) play a critical role after stroke. Many studies dealt with the metabolism of ROS, and there is big knowledge about generation and detoxification of ROS not only after stroke. We developed a new technique of isolated brain perfusion (IBP) without discontinuation of cerebral blood flow during preparation combined with intracranial haemorrhage in a rat-model. The perfusion medium basically consisted of isotonic water without red blood cells or colloids being added. EEG was monitored during IBP, as well as other parameters like temperature, flow rates, arterial pressure or partial press…