Antisense transfection of gene ANK-B inhibits proliferation, invasion and tumorigenicity of Panc1 cells

Introduction: Cytoskeletal protein ankyrin-B (encoded by ANK-B) gene was cloned by RACE method in previous work. It exhibited an overexpression in pancreatic cancer cells and tissues. The antisense ANK-B was introduced into Panc1 cells, resulting in a reduced proliferation, invasion and tumorigenicity of transfected cells. Methods: Rapid amplification of cDNA end (RACE) was used to clone the full-length cDNA of ANK-B. The gene expression was inhibited by transfecting antisense ANK-B into Panc1 cells. The effect on cell proliferation and metastasis of ANK-B silencing was evaluated by WST-1 assay, invasion and mig…