Closure of Fetoscopic Access Sites with Amniotic Extracellular Matrix Scaffolds in an In Vivo Model
N Ochsenbein-Kölble, J Jani, G Verbist, L Lewi, K Marquardt, R Zimmermann, J Deprest · Geburtshilfe und Frauenheilkunde · 2006 · Heft 09
Bibliografische Angaben
Zusammenfassung
Objective: The aim of our study was to develop an amniotic extracellular matrix scaffold for amnion cell in-growth and to evaluate its efficacy in healing a fetoscopic access site in the rabbit model. Material and Methods: Rabbit amnion from one donor doe were treated by enzymatic procedures in order to remove amnion cells from its collagenous extracellular matrix. The acellularity of the amniotic scaffold was investigated by light microcopy, histology and SEM. Fetoscopic access sites were closed with amniotic rabbit scaffolds at 23 days of gestation in 10 rabbit sacs. Additional 10 sacs served as positive contr…
