Multiplex HPV Genotyping of Cervical Scrapes: Comparison of Reverse Line Blot Versus Luminex Technology

Cervical cancer is the second most common cancer in women worldwide. It is causally related to infections with certain, so-called high-risk human papillomavirus (HPV) types. DNA of the known 15 high-risk HPV types can be detected in virtually all cervical cancers. Persistent infection with high-risk types increases the risk for cervical cancer development. HPV types 16 and 18 are most prevalent in cervical cancer, and HPV 16 may have a higher potential for persistence and transformation. Routine screening for precursor lesions is conducted by Papanicolaou staining of exfoliated cervical cells. Type-specific dete…