The macrophage migration inhibitory factor (MIF) –173G/C promoter polymorphism influences upper gastrointestinal tract involvement and disease activity in patients with Crohn's di…
J Dambacher, T Staudinger, J Seiderer, Z Sisic, F Schnitzler, S Pfennig, K Hofbauer, A Konrad, C Tillack, JM Otte, J Diebold, B Göke, T Ochsenkühn, P Lohse, S Brand · Zeitschrift für Gastroenterologie · 2006 · Heft 10
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Zusammenfassung
Objectives: Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine with increased expression in inflammatory bowel disease. Aims: To analyze the role of the MIF –173G/C single nucleotide polymorphism in Crohn's disease (CD). Methods: Using restriction fragment length polymorphism analysis, genomic DNA of 198 patients with CD and of 159 unrelated controls was analyzed for the –173G/C SNP in the MIF promoter region. Colonic MIF mRNA expression was measured by quantitative PCR, serum MIF levels by ELISA. Results: Thirty-six of the 146 G/G wildtype carriers (24.7%), but only three of the 45 G/C h…
