RANK (TNFRSF11A) is epigenetically altered and inactivated in human gliomas
A von dem Knesebeck, J Felsberg, T Mikeska, W Hartmann, A Waha, E Endl, J Hammes, T Pietsch, A Waha · Klinische Pädiatrie · 2009 · Heft 05
Bibliografische Angaben
Zusammenfassung
Alterations of DNA methylation play an important role in carcinogenesis and influence the transcription of tumor suppressor genes in many tumor entities. Using a microarray based technology, we have identified novel candidate genes, which are de novo methylated in gliomas. One of these candidate genes is TNFRSF11A, also known as Receptor Activator of NF-κB (RANK), which after binding of the ligand RANKL activates several signaling pathways e.g.: NF-κB, JNK, ERK, p38 and Akt/PKB. Using bisulfite-sequencing and combined bisulfite restriction analysis (COBRA) we observed RANK methylation in 3 of 9 (33%) astrocytoma…
