The role of the N34S mutation in the trypsin inhibitor gene SPINK1 in patients with chronic pancreatitis from Romania

Introduction. The N34S mutation in the trypsin inhibitor gene SPINK1 was frequently found in patients with early onset idiopathic pancreatitis and in tropical pancreatitis and to a lesser extent in chronic alcoholic pancreatitis. Aim and methods. The aim of the study was to determine the frequency of the N34S mutation in SPINK1 gene in patients with chronic pancreatitis (CP) and controls. We studied 94 patients with chronic pancreatitis (n=80 alcoholic CP, n=10 idiopathic CP, n=4 other causes) and 96 healthy individuals as controls. DNA was isolated from whole blood. The N34S mutation was determined with two met…