New gene expression markers of colorectal adenoma-dysplasia-carcinoma sequence
O Galamb, B Galamb, N Solymosi, F Sipos, S Spisák, K Tóth, B Molnár, Z Tulassay · Zeitschrift für Gastroenterologie · 2007 · Heft 05
Bibliografische Angaben
Zusammenfassung
Background and Aims: Gene expression analysis of colon biopsies using high-density oligonucleotide microarray can contribute to the understanding of local pathophysiological alterations and to functional classification of precancerous adenoma, and different stages of colorectal carcinomas (CRC). As most CRC develop on the basis of villous adenomas, we focused on the gene expression aspects of adenoma-dysplasia-carcinoma sequence. Methods: Total RNA was extracted, amplified and biotinylated from frozen colonic biopsies of 15 patients with CRC, 15 with villous adenoma and 8 normal controls. Genome-wide gene expres…
