CareLit Fachartikel
Activation of latent TGF-β1 within the hepatocyte contributes to connective tissue growth factor (CTGF/CCN2) expression via entirely intracellular signalling
OA Gressner, B Lahme, K Rehbein, M Siluschek, R Weiskirchen, AM Gressner · Zeitschrift für Gastroenterologie · 2008 · Heft 01
Dokument
1076549
CareLit-ID
Jahr
2008
Publikation
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Zusammenfassung
Introduction: TGF-β is regarded as the profibrogenic master cytokine. We aimed at analyzing the regulatory link between TGF-b dependent CTGF-induction and intracellular demasking of latent TGF-b in hepatocytes (PC) of healthy and injured livers. Methods: Primary rat PC were cultured in entirely serum- (i.e. TGF-β-) free conditions. Analyses performed included immunoblotting, reporter-assays, metabolic labelling and immunocytochemistry (with/ without the addition of specific activators/inhibitors of TGF-β signalling) as well as Immunohistochemistry of healthy rat livers and those subjected to bile duct-ligation (…
Schlagworte
CTGF
Smad signaling
TGF-ß1
autocrine signaling
liver fibrosis
Zeitschrift für Gastroenterologie
