Generation of the conditionally replicating adenovirus Adsensor-p53T for the treatment of tumors with harboring activated hTERT and aberrations in p53-transcriptional activity

Oncolytic virotherapy is a novel and challenging approach for the treatment of solid tumors. Major challenges in virotherapy are tight restriction of viral replication to malignant cells combined with a large spectrum of potential target tumors. Aim of the study was the generation of a conditionally replicating adenovirus able to recognize both activated hTert and an aberrant transcriptional p53-status in target cells. The vector should consequently respond by the selective onset of viral replication in tumor cells. Adsensor-p53T was constructed harboring E1A controlled by a GAL4-binding hTERT-promoter and the G…