Influence of intraanalytical and interlaboratory variability on the non-invasive classification of liver fibrosis as determined by calculated Fibrotest/ Actitest scores

Introduction: Non-invasive, i.e. serum-based assessment of liver fibrosis is still an important challenge although multiple single and multiparametric panels of biomarkers have been proposed (1). Aim and Methods: (i) Haptoglobin, ALT, gammaGT, alpha 2-macroglobulin, apolipoprotein A1 and bilirubin in sera of 4 patients with histological proven fibrosis (F1-F4, A1-A3) were determined in 6 different quality-controlled laboratories. Inter-laboratory variations of the calculated Fibrotest Score for staging and Actitest Score for grading (both BioPredictiveTM), and their error ratios compared to the results obtained…