γδ T cells: Stimulators of specific CD8+ T cell responses to sarcoma-associated antigens

Primary metastatic Ewing sarcomas and alveolar rhabdomyosarcomas are often incurable by current state-of-the art treatment. Cellular immunotherapy is a promising complementary strategy to eradicate dormant and chemoresistant tumor cells and prevent sarcoma relapse. We propose that the immunostimulatory properties of activated γδ T cells can be used to induce cytolytic CD8+ T cell responses to the sarcoma-associated self antigens PRAME and STEAP-1. CD8+ T cells from two HLA-A2-positive healthy donors were stimulated with autologous bisphosphonate-activated γδ T cells pulsed with a pool of either four STEAP-1 or f…